abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

journal_title：Letters in applied microbiology

authors： McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

更新日期：2005-01-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：AIMS:The current standard culture methods are unable to detect nongrowing bacteria and, thus, might not be sufficient for precise monitoring of the microbiological quality of waters. The use of a molecular method such as PCR could be a valid alternative to detect bacterial faecal contamination indicators such as Escher...

journal_title：Letters in applied microbiology

authors： Lleo MM,Bonato B,Tafi MC,Signoretto C,Pruzzo C,Canepari P

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...

journal_title：Letters in applied microbiology

authors： Schmidt H,Bannier M,Vogel RF,Niessen L

更新日期：2004-01-01 00:00:00

abstract：AIMS:Dynamic microbial characterization of the colonization of organic carrier during a model biofiltration experiment using methanol as air pollutant. METHODS AND RESULTS:A model biofilter was used in order to characterize the micro-organisms involved in the colonization of a model organic carrier. The model system c...

journal_title：Letters in applied microbiology

authors： Pineda R,Alba J,Thalasso F,Ponce-Noyola T

更新日期：2004-01-01 00:00:00

abstract：AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...

journal_title：Letters in applied microbiology

authors： La Ragione RM,Narbad A,Gasson MJ,Woodward MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

journal_title：Letters in applied microbiology

authors： Laverick MA,Wyn-Jones AP,Carter MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:To evaluate the specificity and sensitivity of PCR primers for the detection of Salmonella enterica in a real-time PCR assay using pure cultures. METHODS AND RESULTS:Unenriched whole cells in sterile water were used as template for each PCR. SYBR Green dye was used for the nonspecific detection of dsDNA. The real...

journal_title：Letters in applied microbiology

authors： Csordas AT,Barak JD,Delwiche MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

journal_title：Letters in applied microbiology

authors： Asis CA Jr,Adachi K

更新日期：2004-01-01 00:00:00

abstract：AIMS:This study aimed to identify a simple, inexpensive preservation technique that will allow a quick and reliable recovery of Campylobacter jejuni following long-term periods of preservation. METHODS AND RESULTS:Preservation techniques include (i) Cryobank microbial preservation system using hypertonic 'cryopreserva...

journal_title：Letters in applied microbiology

authors： Gorman R,Adley CC

更新日期：2004-01-01 00:00:00

abstract：AIMS:Immunologically based assays for the detection of staphylococcal enterotoxins are numerous. These techniques include radio immunosorbent assays and enzyme-linked immunosorbent assays (ELISA), some of which are available as commercial kits. The purpose of this study was to compare the performances of three commerci...

journal_title：Letters in applied microbiology

authors： Vernozy-Rozand C,Mazuy-Cruchaudet C,Bavai C,Richard Y

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were ...

journal_title：Letters in applied microbiology

authors： Ramabu SS,Boxall NS,Madie P,Fenwick SG

更新日期：2004-01-01 00:00:00

abstract：AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...

journal_title：Letters in applied microbiology

authors： Arshad M,Nazli ZH,Khalid A,Zahir ZA

更新日期：2004-01-01 00:00:00

abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

journal_title：Letters in applied microbiology

authors： Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

更新日期：2004-01-01 00:00:00

abstract：AIMS:To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis. METHODS AND RESULTS:Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supple...

journal_title：Letters in applied microbiology

authors： Jung YS,Anderson RC,Callaway TR,Edrington TS,Genovese KJ,Harvey RB,Poole TL,Nisbet DJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS:The N-terminal fusion expression of EGFP...

journal_title：Letters in applied microbiology

authors： Roh JY,Li MS,Chang JH,Choi JY,Shim HJ,Shin SC,Boo KS,Je YH

更新日期：2004-01-01 00:00:00

abstract：AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...

journal_title：Letters in applied microbiology

authors： He GQ,Kong Q,Ding LX

更新日期：2004-01-01 00:00:00

abstract：AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...

journal_title：Letters in applied microbiology

authors： Westers H,Darmon E,Zanen G,Veening JW,Kuipers OP,Bron S,Quax WJ,van Dijl JM

更新日期：2004-01-01 00:00:00

abstract：AIMS:This paper attempts to develop a kinetic model to describe the growth of aerobic granules developed under different operation conditions. METHODS AND RESULTS:A series of experiments were conducted by using four-column sequencing batch reactors to study the formation of aerobic granules under different conditions,...

journal_title：Letters in applied microbiology

authors： Yang SF,Liu QS,Tay JH,Liu Y

更新日期：2004-01-01 00:00:00

abstract：AIMS:To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. METHODS AND RESULTS:Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was s...

journal_title：Letters in applied microbiology

authors： de la Fuente G,Cebrián JA,Fondevila M

更新日期：2004-01-01 00:00:00

abstract：AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...

journal_title：Letters in applied microbiology

authors： Chen JC,Lin ZH,Ma XX

更新日期：2003-01-01 00:00:00

abstract：AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

journal_title：Letters in applied microbiology

authors： Chowdhury B,Adak M,Bose SK

更新日期：2003-01-01 00:00:00

abstract：AIMS:Nine sets of PCR primers targeting Salmonella were evaluated for their specificity with pure cultures of intestinal-associated bacteria prior to their application to Salmonella detection in faecal samples. METHODS AND RESULTS:Gene targets of PCR primers included: 16S rDNA, a Salmonella pathogenicity island I viru...

journal_title：Letters in applied microbiology

authors： Ziemer CJ,Steadham SR

更新日期：2003-01-01 00:00:00

abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...

journal_title：Letters in applied microbiology

authors： Fitzgerald AC,Edrington TS,Looper ML,Callaway TR,Genovese KJ,Bischoff KM,McReynolds JL,Thomas JD,Anderson RC,Nisbet DJ

更新日期：2003-01-01 00:00:00

abstract：AIMS:The study evaluated the efficacy of four Mycobacterium avium subsp. paratuberculosis (MAP) culture media in suppressing commonly used starter cultures and typical nonstarter microflora present during the manufacture and ripening of Cheddar cheese, with a view to identify a suitable medium for the enumeration of MA...

journal_title：Letters in applied microbiology

authors： Donaghy JA,Totton NL,Rowe MT

更新日期：2003-01-01 00:00:00

abstract：AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...

journal_title：Letters in applied microbiology

authors： Taylor RK,Hale CN

更新日期：2003-01-01 00:00:00

abstract：AIMS:A simplified and rapid method was developed to identify and enumerate total mesophilic microbial load, gram-negative bacteria, coliforms, and Escherichia coli in ground beef, using a single module. METHODS AND RESULTS:A 96-well microtiter plate was divided into four sections of two lanes (24 wells), with each sec...

journal_title：Letters in applied microbiology

authors： Kang DH,Rhee MS,Costello M

更新日期：2003-01-01 00:00:00

abstract：AIMS:A commercial biochemical panel ID kit was used to identify presumptive enterococci isolates of veterinary or agricultural origin obtained during different steps of culture. METHODS AND RESULTS:Fifty isolates identified as enterococci using a genus PCR assay were tested for genus and species identification using t...

journal_title：Letters in applied microbiology

authors： Hudson CR,Fedorka-Cray PJ,Jackson-Hall MC,Hiott LM

更新日期：2003-01-01 00:00:00

abstract：AIMS:To provide evidence that DNA-PCR diagnostics of oral pathogens based on standard sample collection by paper point insertion from the depth of the periodontal pocket can be replaced by a novel non-invasive collection method based on swab technique from the gingiva. METHODS AND RESULTS:In this study we compared the...

journal_title：Letters in applied microbiology

authors： Smola SF,Rettenberger G,Simmet T,Burysek L

更新日期：2003-01-01 00:00:00

abstract：AIMS:The objective of the study was to evaluate the variability of germination response of 10 strains of proteolytic Clostridium botulinum. METHODS AND RESULTS:An automated turbidometric method was used to follow the fall in optical density. Spores of proteolytic Cl. botulinum germinated in response to l-alanine alone...

journal_title：Letters in applied microbiology

authors： Alberto F,Broussolle V,Mason DR,Carlin F,Peck MW

更新日期：2003-01-01 00:00:00

abstract：AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

journal_title：Letters in applied microbiology

authors： Zanetti F,De Luca G,Tarlazzi P,Stampi S

更新日期：2003-01-01 00:00:00

abstract：AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...

journal_title：Letters in applied microbiology

authors： Rodríguez E,Arqués JL,Rodríguez R,Nuñez M,Medina M

更新日期：2003-01-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...

journal_title：Letters in applied microbiology

authors： Richard C,Brillet A,Pilet MF,Prévost H,Drider D

更新日期：2003-01-01 00:00:00

abstract：AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...

journal_title：Letters in applied microbiology

authors： Myers JM,Myers CR

更新日期：2003-01-01 00:00:00

abstract：AIMS:To determine the effect of interacting conditions of water activity (aw, 0.99-0.85), temperature (15, 25 degrees C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat-based agar medium. METHODS AND RESULTS:Fusarium culmorum grew o...

journal_title：Letters in applied microbiology

authors： Hope R,Magan N

更新日期：2003-01-01 00:00:00

abstract：:Phorate [O,O-diethyl-S-(ethylthio)methyl phosphoradiothioate] degrading bacteria were isolated from agricultural soil and characterized based on their morphological and biochemical characteristics. The selected isolates PS-1, PS-2 and PS-3 were presumptively identified as Rhizobium, Pseudomonas and Proteous species, r...

journal_title：Letters in applied microbiology

authors： Bano N,Musarrat J

更新日期：2003-01-01 00:00:00

abstract：AIMS:To identify the abattoir source(s) of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats. METHODS AND RESULTS:Molecular procedures were used to detect the presence of specific 16S rRNA gene fragments of blown pack-causing clostridia in samples collected from a commercial abattoi...

journal_title：Letters in applied microbiology

authors： Boerema JA,Broda DM,Bell RG

更新日期：2003-01-01 00:00:00

abstract：AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...

journal_title：Letters in applied microbiology

authors： Tana,Watarai S,Isogai E,Oguma K

更新日期：2003-01-01 00:00:00

abstract：AIMS:To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina. METHODS AND RESULTS:Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production...

journal_title：Letters in applied microbiology

authors： Xian M,Nie J,Meng Q,Liu J,Zhou C,Kang Y,Zhen K

更新日期：2003-01-01 00:00:00

abstract：AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end ...

journal_title：Letters in applied microbiology

authors： Carducci A,Verani M,Casini B,Giuntini A,Mazzoni F,Rovini E,Passaglia A,Giusti L,Valenza A,Lombardi R

更新日期：2002-01-01 00:00:00